Journal: Applied Microbiology and Biotechnology

Article Title: Making GFP count: a validated framework for absolute protein quantification in precision fermentation

doi: 10.1007/s00253-026-13734-z

Figure Lengend Snippet: SDS-PAGE gel of culture samples E. coli BL21 pRSET_J23108_EmGFP (replicate 1 as an example). Lane 1, molecular weight marker 250–10 kDa; lanes 2–7, time points t6–t11 (t6 after 12 h, t7 after 14 h, t8 after 16 h, t9 after 19 h, t10 after 22 h, t11 after 24 h); lane 8, commercial GFP standard (26.9 kDa); lane 9, in-house EmGFP standard (26.9 kDa). The analysis table presents corresponding EmGFP lane intensities (%) and calculated EmGFP concentration in mg/L for time points t6–t11

Article Snippet: Standards and references listed in Table used in this work were commercially purchased (sodium fluorescein [Na-F*], supplier: Carl Roth, GFP supplier: Merck) or prepared in-house (EmGFP, see section “ In-house EmGFP standard preparation”).

Techniques: SDS Page, Molecular Weight, Marker, Concentration Assay